Cargo Conicet: Investigador Independiente
Área: Biología Molecular y Celular
Tema de Trabajo: Estrategias y mecanismos de evasión inmune empleados por Brucella abortus
Descripción de trabajo: Las bacterias del género Brucella son el agente etiológico de la brucelosis. B. abortus es un patógeno intracelular capaz de sobrevivir dentro de macrófagos. Sin embargo, B. abortus puede persistir dentro de los mismos, estableciendo una infección crónica. El objetivo general de nuestro grupo es estudiar las estrategias y mecanismos de evasión empleados por B. abortus sobre los monocitos/macrófagos y linfocitos T, para evadir la vigilancia inmunológica y persistir crónicamente en el hospedador.

1. Milillo MA, Trotta A, Serafino A, Marinho FV, Alcain J, Genoula M, Balboa L, Oliveira SC, Giambartolomei GH, Barrionuevo P. Brucella abortus RNA contributes to the down-modulation of MHC-II expression on monocytes/macrophages diminishing CD4+ T cell responses. Milillo MA, Trotta A, Serafino A, Marin Franco JL, Marinho FV, Alcain J, Genoula M, Balboa L, Oliveira SC, Giambartolomei GH, Barrionuevo P. Frontiers in Immunology, 10: 2182, 2019. doi: 10.3389/fimmu.2019.02181.

2. Barrionuevo P and Giambartolomei GH. Inhibition of antigen presentation by Brucella: many more than many ways. Review. Microbes and Infection, pii: S1286-4579(19)30002-4, 2019.

3. Trotta A, Velásquez LN*, Milillo MA*, Delpino MV, Rodríguez AM, Landoni VI, Giambartolomei GH, Pozner RG, Barrionuevo P. Platelets promote Brucella abortus monocyte invasion by establishing complexes with monocytes. Frontiers in Immunology, 9: 1000, 2018. *These authors contributed equally to this work. doi: 10.3389/fimmu.2018.01000.

4. Milillo MA, Velásquez LN, Trotta A, Delpino MV, Marinho FV, Balboa L, Vermeulen M, Espindola S, Rodriguez-Rodrigues N, Fernández GC, Oliveira SC, Giambartolomei GH, Barrionuevo P. B. abortus RNA is the component involved in the down-modulation of MHC-I expression on human monocytes via TLR8 and the EGFR pathway. PLoS Pathogens, 13(8): e1006527, 2017.

5. Velásquez LN*, Milillo MA*, Delpino MV, Trotta A, Mercogliano MF, Pozner RG, Schillaci R, Elizalde PV, Giambartolomei GH, Barrionuevo P. Inhibition of MHC-I by Brucella abortus is an early event during infection and involves EGFR pathway. Immunology and Cell Biology, 95(4): 388-398, 2017. *These authors contributed equally to this work.

6. Velásquez LN, Milillo MA, Delpino MV, Trotta A, Fernández P, Pozner RG, Lang R, Balboa L, Giambartolomei GH, Barrionuevo P. Brucella abortus down-regulates MHC class II by the IL-6-dependent inhibition of CIITA through the down-modulation of IFN regulatory factor-1 (IRF-1). Journal of Leukocyte Biology, 101(3): 759-773, 2017.

7. Barrionuevo P, Delpino MV, Pozner RG, Velásquez LN, Cassataro J, Giambartolomei GH. Brucella abortus induces intracellular retention of MHC-I molecules in human macrophages down-modulating cytotoxic CD8+ T cell responses. Cellular Microbiology, 15(4): 487-502, 2013. Este artículo fue elegido como “Editors Choice” y fue destacado por ello en el volumen de abril de 2013 de la Revista Cellular Microbiology.

8. Velásquez LN, Delpino MV, Ibañez AE, Coria LM, Miraglia MC, Scian R, Cassataro J, Giambartolomei GH, Barrionuevo P. Brucella abortus induces apoptosis of human T lymphocytes. Microbes and Infection, 14: 639-50, 2012.

9. Barrionuevo P, Delpino MV, Velásquez LN, García Samartino C, Coria LM, Ibañez AE, Rodríguez ME, Cassataro J, Giambartolomei GH. Brucella abortus inhibits IFN-γ-induced FcγRI expression and FcγRI-restricted phagocytosis via Toll-Like Receptor 2 on human monocytes/macrophages. Microbes and Infection, 13(3): 239-50, 2011.

10. Barrionuevo P, Cassataro J, Delpino MV, Zwerdling A, Pasquevich KA, García Samartino C, Wallach JC, Fossati CA, Giambartolomei GH. Brucella abortus inhibits major histocompatibility complex class II expression and antigen processing through interleukin-6 secretion via Toll-like receptor 2. Infection and Immunity, 76(1): 250-62, 2008.

11. Barrionuevo P, Beigier-Bompadre M, Ilarregui JM, Toscano MA, Isturiz MA, Rabinovich GA. A novel function for galectin-1 at the crossroad of innate and adaptive immunity: galectin-1 regulates monocyte/ macrophage physiology through a non-apoptotic ERK-dependent pathway. Journal of Immunology, 178: 436-45, 2007.

12. Barrionuevo P, Beigier-Bompadre M, Fernández GC, Gómez S, Alves-Rosa MF, Fernández G, Palermo MS, Isturiz MA. Immune complex-FcR interaction modulates monocyte/ macrophage molecules involved in inflammation and immune-response. Clinical and Experimental Immunology, 133(2): 200-7, 2003.

13. Barrionuevo P, Beigier-Bompadre M, de la Barrera S, Alves-Rosa MF, Fernández G, Palermo MS, Isturiz MA. Immune complexes (IC) down-regulate the basal and interferon--induced expression of MHC class II on human monocytes. Clinical and Experimental Immunology, 125(2): 251-7, 2001.