Cargo Conicet: Investigador Independiente
Área: Biología Molecular y Celular
Tema de Trabajo: Regulación génica en células madre
Descripción de trabajo: Las células madre pluripotentes son capaces de propagarse indefinidamente y de diferenciarse a todos los tipos celulares del organismo, recapitulando las cualidades de una célula primitiva presente en un embrión. Estas células son un interesante modelo para el estudio de la diferenciación y tienen un gran potencial en el área de la medicina regenerativa. Nos enfocamos en el estudio de los mecanismos moleculares involucrados en el mantenimiento de las propiedades fundamentales de estas células.

-Solari C, Petrone MV, Toro A, Vázquez Echegaray C, Cosentino MS, Waisman A, Francia M, Barañao L, Miriuka S and Guberman A. The pluripotency transcription factor Nanog represses Glutathione reductase gene expression in mouse embryonic stem cells BMC Research Notes 2019 Jul 1;12(1):370. doi: 10.1186/s13104-019-4411-0. ISSN: 1756-0500 (Online). https://rdcu.be/bIjm5

-Waisman, A., Sevlever F., Costa, M., Cosentino, S., Miriuka, S., Ventura, A., Guberman A. Cell cycle dynamics of mouse embryonic stem cells in the ground state and during transition to formative pluripotency. Scientific Reports (2019). 9 (1):8051, https://doi.org/10.1038/s41598-019-44537-0

-Cosentino MS, Oses C, Vázquez Echegaray C, Solari C, Waisman A, Álvarez Y, Petrone MV, Francia M, Schultz M, Sevlever G, Miriuka S, Levi V, Guberman A. Kat6b Modulates Oct4 and Nanog Binding to Chromatin in Embryonic Stem Cells and Is Required for Efficient Neural Differentiation. J Mol Biol. (2019) Feb 18. pii: S0022-2836(19)30088-9. doi: 10.1016/j.jmb.2019.02.012

-Waisman A, La Greca A, Möbbs AM, Scarafía MA, Santín Velazque NL, Neiman G, Moro LN, Luzzani C, Sevlever GE, Guberman AS, Miriuka SG. Deep learning neural network highly predicts very early onset of pluripotent stem cell differentiation. Stem Cell Reports (2019) Feb 28. pii: S2213-6711(19)30052-9. doi: 10.1016/j.stemcr.2019.02.004

-Canizo J., Vazquez Echegaray C., Klisch D., Aller J., Paz D., Alberio RH. Alberio R., Guberman A. Exogenous human OKSM Factors Maintain Pluripotency Gene Expression of Bovine and Porcine iPS-like cells Obtained with STEMCCA Delivery System BMC Research Notes (2018) 11 (1):509.
https://doi.org/10.1186/s13104-018-3627-8

-Solari, C, Petrone, MV, Vázquez Echegaray, C, Cosentino, MS, Waisman, A, Francia, M, Barañao, L, Miriuka, S, Guberman, A. Superoxide dismutase 1 expression is modulated by the core pluripotency transcription factors Oct4, Sox2 and Nanog in embryonic stem cells. (2018). Mechanisms of Development. (2018) 154. 116-121. Jun 19. pii: S0925-4773(18)30055-8. doi: 10.1016/j.mod.2018.06.004.

– Verneri P, Romero J, De Rossi MC, Alvarez Y, Oses C, Guberman A *, Levi V*. Imaging transcription factors dynamics with advanced fluorescence microscopy Methods (2018) Review. Mechanisms of Development. (2018) 154. 60-63. May 14. pii: S0925-4773(18) 30008-X. doi: 10.1016/j.mod.2018.05.003.

-Waisman, A., Vazquez Echegaray, C., Solari, C., Cosentino, S., Martyn, I., Deglincerti, A., Zeeshan Ozair, M., Ruzo Matias, A., Barañao, L., Miriuka, S., Brivanlou, A, Guberman, A. DNA replication is required for transcriptional switch during naïve pluripotency exit. (2017) Journal of Molecular Biology. Sep 1;429(18):2802-2815. doi: 10.1016/j.jmb.2017.06.020. Este trabajo es tapa de Journal of Molecular Biology: https://www.journals.elsevier.com/journal-of-molecular-biology/cover-gallery/cover-of-volume-429-issue-24

-L Romorini, X Garate, G Neiman, C Luzzani, V Furmento, A Guberman, G Sevlever, M Scassa and S Miriuka AKT/GSK3β signaling pathway is critically involved in human pluripotent stem cell survival. (2016) Scientific Reports; 6: 35660. doi: 10.1038/srep35660. ISSN 2045-2322

-Solari, C., Vázquez Echegaray, C., Cosentino, MS., Waisman, A., Petrone, MV., Francia, M., Sassone, A., Canizo, J., Sevlever, G., Barañao, L., Miriuka, S., Guberman, A. Protein Arginine Methyltransferase 8 gene is expressed in pluripotent stem cells and its expression is modulated by the transcription factor Sox2. Biochemical and Biophysical Research Communications. (2016) Apr 22; 473 (1): 194-9. doi: 10.1016/j.bbrc.2016.03.077. Epub 2016 Mar 21. ISSN: 0006-291X

-Questa M, Romorini L, Blüguermann C, Solari C, Neiman G, Luzzani C, Scassa ME, Sevlever G, Guberman A, Miriuka S. Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human Foreskin fibroblasts. Stem Cell Research (16) 2016: 300-303. ISSN: 1873-5061

-Claudia Solari, Camila Vázquez Echegaray, María Soledad Cosentino, María Victoria Petrone, Ariel Waisman, Carlos Luzzani, Marcos Francia, Emilly Villodre, Guido Lenz, Santiago Miriuka, Lino Barañao and Alejandra Guberman. Manganese Superoxide Dismutase Gene Expression Is Induced by Nanog and Oct4, Essential Pluripotent Stem Cells’ Transcription Factors. PLOS ONE | DOI:10.1371/journal.pone.0144336 Diciembre, 2015 (1-12). ISSN: 1932-6203.

-Luzzani C, Neiman G, Garate X, Questa M, Solari C, Fernandez Espinosa D, García M, Errecalde AL, Guberman A, Scassa ME, Sevlever GE, Romorini L, Miriuka SG. A therapy-grade protocol for differentiation of pluripotent stem cells into mesenchymal stem cells using platelet lysate as supplement. Stem Cell Res Ther. 2015 Jan 12;6 (1):6. ISSN: 1757-6512

-Losino, Waisman, Solari, Luzzani, Fernandez Espinosa, Sassone, Muro, Miriuka, Sevlever, Barañao, and Guberman EDA-containing fibronectin increases proliferation of embryonic stem cells. PLoS One. 2013 Nov 14; 8(11):e80681. doi: 10.1371/journal.pone.0080681.ISSN: 1932-6203.

-Carlos Luzzani; Claudia Solari; Noelia Losino; Ariel Waisman; Leonardo Romorini; Carolina Bluguermann; Gustavo Sevlever; Lino Barañao; Santiago Miriuka; Alejandra Guberman (2011). Modulation of chromatin modifying factors´ gene expression in embryonic and induced pluripotent stem cells. Biochemical and Biophysical Research Communications. 2011 Jul 15; 410 (4): 816-22. Epub 2011 Jun 15. ISSN: 0006-291X

-Claudia Solari; Noelia Losino; Carlos Luzzani; Ariel Waisman; Carolina Bluguermann; Maria Questa; Gustavo Sevlever; Santiago Miriuka; Lino Barañao; Alejandra Guberman (2011). Induced Pluripotent Stem Cells’ self-renewal and pluripotency is maintained by a bovine granulosa cell line-conditioned medium. Biochemical and Biophysical Research Communications. 2011 Jul 1; 410 (2): 252-7. Epub 2011 May 30. ISSN: 0006-291X

-Losino N., Luzzani, C., Solari, C., Boffi, J., Louis Tisserand, M., Sevlever, G., Barañao, L. and Guberman, A. (2010). Maintenance of Murine Embryonic Stem Cells’ Self-Renewal and Pluripotency with Increase in Proliferation Rate by a Bovine Granulosa Cell Line-Conditioned Medium. Stem Cells and Development. 2011 Ago, 20 (8): 1439-1449. Epub 2011 Jan 12. Online Ahead of Editing: December 2, 2010. DOI: 10.1089, cd.2010.0336. ISSN: 1547-3287 • Monthly • Online ISSN: 1557-8534